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Measured performance, by plate and liquid

Each card shows how the VolumeSense® instrument performs on one microplate type (96- and 384-well; flat-bottom, round-bottom and PCR) with one liquid: how closely its volumes match gravimetry, and how repeatably it reads the same well. It also shows the well-to-well volume variation the instrument measured on those plates after bulk filling. If your plates and liquids are listed, these numbers show what to expect from the instrument in liquid handler verification.

How we measure accuracy ↓

Characterized so far

Plates and liquids

    More plates and liquids are added as they are calibrated. Don’t see yours? Send us your plate →

    Liquids used on the cards
    70% ethanolethanol 70 % v/v in water
    DI waterdeionized water
    PBS137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, pH 7.4
    PBS + 0.02% TweenPBS with 0.02 % v/v Tween 20
    PBS + 0.05% TweenPBS with 0.05 % v/v Tween 20
    PBS + 40 mg/mL BSAPBS with 40 mg/mL bovine serum albumin
    Potassium phthalate bufferpH 6.00 buffer solution: potassium hydrogen phthalate 1.08 %, sodium hydroxide 0.19 %, in water
    TE buffer10 mM Tris, 1 mM EDTA, pH 8.0
    Watermunicipal tap water

    Reading a card

    Three numbers per plate and liquid

    AccuracyHow close the instrument’s plate-mean volume comes to gravimetry on a level or a plate the calibration never saw: FRESH, or FRESH², explained below.
    RepeatabilityHow closely the instrument reads the same well again, with the plate left in place.
    Plate CVHow much the volume varies across the wells of a filled plate, from the dispenser, the plate and the instrument together, split into those parts where the data allow.

    The method behind every number is described in our paper (forthcoming).

    How we measure accuracy

    FRESH

      • Calibration point
      • Left out, predicted
      • Second plate
      • Prediction error (volume)
      Step 1 of 6

      Illustration, not measured data. Errors are magnified so they can be seen; on real plates they are a small fraction of a microliter.

      Don’t see your plate?

      Send us your labware, your liquid, or both. We’ll calibrate it and share what we find.

      Send us a plate